Coding
Part:BBa_K5154006:Design
Designed by: Zijiao Wang Group: iGEM24_Cambridge (2024-09-28)
TEV-F5-EPG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 654
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 654
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 704
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 654
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 654
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Remove cut site, linker optimization
Source
Kryptopterus bicirrhis, Nicotiana tabacum