Coding

Part:BBa_K5154006:Design

Designed by: Zijiao Wang   Group: iGEM24_Cambridge   (2024-09-28)


TEV-F5-EPG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 654
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 704
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 654
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 654
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Remove cut site, linker optimization


Source

Kryptopterus bicirrhis, Nicotiana tabacum

References